Research Paper Volume 14, Issue 10 pp 4556—4571

Figure 3. Correlations of ALDH7A1 rs13182402 genotypes with ALDH7A1 mRNA levels in five oral cancer cell lines. (A) Upper panel, ALDH7A1 rs13182402 genotypes in oral cancer cell lines (SCC-14, SAS, CA9-22, HSC-3, and OECM-1) were detected by a TaqMan SNP Genotyping Assay. Lower panel, mRNA level of ALDH7A1 was detected by quantitative real time-PCR analysis. (B) The migratory ability in oral cancer cell lines (SCC-14, SAS, CA9-22, HSC-3, and OECM-1) was detected by Boyden chamber migration assays. (C) Western blot analysis and real time-PCR assay for ALDH7A1 protein and mRNA expressions of SAS cells after siRNA directly against the ALDH7A1 expression were conducted. (D) Western blot analysis and real time-PCR assay for ALDH7A1 protein and mRNA expressions of CA9-22 cells after transfection with vectors containing a constitutively active ALDH7A1 cDNA were conducted. (E and F) Boyden chamber migration assays for cell migratory ability in SAS cells and CA9-22 cells were conducted.