Research Paper Volume 14, Issue 10 pp 4572—4585
Lactotransferrin promotes intervertebral disc degeneration by regulating Fas and inhibiting human nucleus pulposus cell apoptosis
Figure 4. Human primary NPC identification. (A) The general morphology of primary NPCs, which mainly were star-shaped, long fusiform, polygonal or irregular under the microscope; (B) Safranin-O fast green staining: NPCs nucleus is stained dark red, cytoplasm is lightly stained; (C) Toluidine blue staining: NPCs were dyed indigo blue, and the nucleus is located in the center of the cell or tilted to one side; (D) Immunofluorescence staining: The fluorescence of type II collagen is widely expressed in NPCs, most of which are located in the cytoplasm. The closer to the nucleus, the stronger the fluorescence intensity.