Research Paper Volume 14, Issue 14 pp 5628—5640

Figure 4. Effects of resveratrol treatment on mitochondrial functions in oocytes derived from aging mice. MII oocytes derived from aging mice without (control) or with one week of resveratrol (Res) treatment and young animals without resveratrol treatment (young) were used for different mitochondrial assays. (A and B) Mitochondrial membrane potential. (A) Representative fluorescence images showing mitochondrial membrane potential visualized by MitoTracker™ dye (orange). Oocyte nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm. (B) The fluorescence intensities of mitochondrial membrane potential. The intensity of mitochondrial fluorescence in ooplasm of MII oocyte was measured by excluding that in the first polar body (control: n = 25, Res: n = 15 and young: n = 26 oocytes). (C) The ATP levels in MII oocytes. The ATP level per MII oocyte was measured using the ATP-Glo™ Bioluminometric Cell Viability Assay Kit (control: n = 24, Res: n = 16 and young: n = 17 oocytes). (D) The mitochondrial DNA (mtDNA) copy numbers of MII oocytes (10 oocytes from each group). The copy number was measured by absolute real-time RT-PCR. (control: n = 11, Res: n = 13 and young: n = 18 groups). Bars represent means ± SE. ^{A, B}p < 0.05 vs. controls.