Research Paper Volume 14, Issue 16 pp 6520—6536
Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
Figure 1. Dicer is significantly downregulated in highly invasive breast cancer cells. (A, B) A panel of highly invasive breast cancer (MCF-7/I-3 and MDA-MB-231/I-3) cells was established, and their migration and invasion abilities were evaluated by performing cell migration and invasion assays, respectively. (C, D) The protein expression levels of Dicer in highly invasive cells (MCF-7/I-3 and MDA-MB-231/I-3) and parental cells (MCF-7 and MDA-MB-231). (E, F) Analysis of miRNA biogenesis–related protein expression levels in highly invasive cells (MCF-7/I-3 and MDA-MB-231/I-3) cells compared with those in parental cells (MCF-7 and MDA-MB-231) through Western blotting. Data are presented as the mean ± standard error mean of three independent experiments. ***P < 0.001.