Research Paper Volume 14, Issue 16 pp 6520—6536

Figure 3. Dicer silencing enhances the migration/invasion, and CSCs properties of breast cancer cells. (A) The stable Dicer-silenced MCF-7 and MDA-MB-231 cells were analyzed through Western blotting. (B) The migration and invasion of the stable knockdown breast cancer cells were evaluated by performing cell migration and invasion assays, respectively. (C, D) qRT-PCR analysis of Oct-4, Nanog, SOX-2, and KLF4 in Dicer-knockdown MCF-7 and MDA-MB-231 cells. (E) Analysis of ALDH activity through flow cytometry to determine the subpopulation of CSCs properties. (F) Sphere formation assay to evaluate self-renewal and differentiation at the single-cell level in vitro in the present cells. Data are presented as the mean ± standard error mean of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.