Research Paper Volume 14, Issue 16 pp 6520—6536

Figure 5. miR-200b is involved in Dicer-mediated cell migration/invasion, and CSCs properties of breast cancer cells. (A) Analysis of miR-200b, miR-200c, miR-21, miR-203, miR-205, and miR-128 in MCF-7/shDicer cells compared with MCF-7 cells through quantitative real-time polymerase chain reaction (qRT-PCR). (B) qRT-PCR analysis was performed to detect miR-200b levels in the MCF-7/shDicer cells transfected with a miR-200b mimic. (C) The migration and invasion of the indicated cells were examined using the cell migration and invasion assays, respectively. (D) The protein expression levels of E-cadherin, N-cadherin, Vimentin, Snail, and Zeb1 were analyzed through Western blot. (E) The expression levels of Oct-4, Nanog, SOX-2, and KLF4 mRNA were analyzed through qRT-PCR. (F) Analysis of ALDH activity to determine the subpopulation of CSCs properties of the present cells. (G) Sphere formation was examined to measure the self-renewal ability of MCF-7 cells with Dicer knockdown and miR-200b overexpression. Data are presented as the mean ± standard error mean of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.