Research Paper Volume 14, Issue 16 pp 6427—6448
An aqueous extract of the brown alga Eisenia bicyclis extends lifespan in a sex-specific manner by interfering with the Tor-FoxO axis
Figure 5. Mode of action of the Eisenia bicyclis extract induced lifespan prolongation. The alpha-Amylase activity of control flies and EBE treated ones (A) are displayed. Lifespan analyses of control and EBE-treated female flies of the genotypes: dSir2-deficient (B), TOR-deficient (C), and FoxO-deficient (D). Western blot analysis of samples from control flies (Co) and EBE-treated ones (EBE) of puromycin treated flies (E) and the quantification of bad intensities (F). lifespan did not increase on concentrated medium containing 0.05 % EBE, (n > 100 per condition). Protein synthesis of flies treated with 0.05 % EBE was not affected (E, F). N≥10, mean values ± S.E.M. are given (A, F). Statistical analyses were performed with unpaired t-tests. ns means not significant. (B–D, n > 100 per condition). Statistical analyses were done using a log-rank test. ns means not significant, **** means p<0.001.