Research Paper Volume 14, Issue 16 pp 6594—6604

Figure 4. MiR-375 negatively regulates the expression of JAK2 by inhibiting its translation. (A) The potential binding site for miR-375 in the 3’UTR of JAK2. (B) miR-375 significantly decreased the luciferase activity of the JAK2 WT plasmid, while no detectable effects were observed on the luciferase activity of the MT plasmid. (C, D) miR-375 had no detectable effects on the mRNA expression of JAK2 (C), but significantly depressed its protein expression (D). (E) The mRNA expression of JAK2 was not affected by anti-miR-375. (F) Treatment with anti-miR-375 induced the protein expression of JAK2. All values are represented as the mean ± standard error of the mean. n = 3 per group. *P < 0.05.