Research Paper Volume 14, Issue 16 pp 6594—6604

Figure 6. STAT3 is involved in miR-375-regulated cell proliferation in RMECs. (A) The phosphorylation of STAT3 was induced by anti-miR-375, whereas miR-375 repressed the phosphorylation of STAT3. There were no significant changes on the expression of total STAT3 after the treatment with miR-375 or anti-miR-375. The expression of p-STAT3 was quantified by the ratio of p-STAT3 to STAT3 and the expression of STAT3 was quantified by the ratio of STAT3 to β-actin. (B, C) The increase in cell viability (B) and BrdU incorporation (C) induced by anti-miR-375 was mitigated by the inhibition of STAT3. * P < 0.05 vs. anti-miR-375+Stattic group. (D, E) Anti-miR-375-increased PCNA expression (D) and cell migration (E) was attenuated by blocking the STAT3 pathway. Scale bar: 100 μm. All values are represented as the mean ± standard error of the mean. n = 3 per group. *P < 0.05.