Research Paper Volume 14, Issue 16 pp 6626—6641

Figure 4. Oxypeucedanin inhibited the activation of the MAPK signaling pathway in LPS-induced mice and LPS-induced RAW264.7 cells. (A) Western blotting was used to analyze the protein of the MAPK signaling pathway in RAW264.7 cells (n=3). Quantitative analysis of the protein levels in the MAPK signaling pathway by Image J. (B) Western blotting was used to analyze the protein of the MAPK signaling pathway in LPS-induced mice(n=3). Quantitative analysis of protein in MAPK signaling pathway by Image J. The concentrations of OPD in cell and animal experiments were 12.5μM and 15mg/kg, respectively. SEM was used as the error standard for data analysis, and the experiment was repeated three times independently. ^#p < 0.01 and ^##p < 0.0001 compared with No-treatment group; ^**p < 0.01, ^***p < 0.001 and ^****p < 0.0001 compared with the LPS group. LPS: Lipopolysaccharide; OPD: Oxypeucedanin; NT: No-treatment group.