Research Paper Volume 14, Issue 16 pp 6642—6655

Figure 2. Effects of TRAIL on cell viability and apoptosis in 5637, T24, BIU 87, and SV-HUC-1 cells. (A) Bladder epithelial cell (SV-HUC-1) and bladder cancer cell lines (5637, T24 and BIU 87) were treated with different concentrations of TRAIL (0, 5, 10 and 20 ng/mL) for 24 h, and cell viabilities were detected by CCK8 kit. (B and C) According to the sensitivity of bladder cells to TRAIL, 5637 cells were treated with 5 ng/mL TRAIL, T24, BIU 87 and SV-HUC-1 cells were treated with 20 ng/mL TRAIL. After 24 h of treatment, apoptosis was detected using the Annexin V-FITC/PI apoptosis kit. The ANOVA followed by multiple pairwise comparisons using a one-way Student’s t-test procedure was used to analyze differences between treatment and control groups. The results represent the means ± SD of three replicates, ^**P < 0.01, ^***P < 0.001.