Research Paper Volume 14, Issue 16 pp 6642—6655

Figure 3. Cell viability and apoptosis in 5637, T24, BIU 87, and SV-HUC-1 cells by treated with RH. (A) After being treated with different concentrations of RH (0, 5, 10, 20 and 40 μg/mL) for 24 h, cell viability of 5637, T24, BIU 87 and SV-HUC-1 cells was measured with CCK8. (B and C) Treated with RH (10 μg/mL) for 24 h, cell apoptosis of 5637, T24, BIU 87 and SV-HUC-1 were detected using FCM. The ANOVA followed by multiple pairwise comparisons using a one-way Student’s t-test procedure was used to analyze differences between treatment and control groups. The results represent the means ± SD of three replicates, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.