Research Paper Volume 14, Issue 16 pp 6642—6655

Figure 5. The changes of DR5 and DR4 in bladder cancer cells by treated with RH. (A) The cell surface DR5 and DR4 of T24, 5637 and BIU 87 cells were measured via FCM after treatment with RH (10 μg/mL) for 24 h. (B) TRAIL-sensitive cells (5637) and TRAIL-resistant cells (T24 and BIU 87) were treated with different concentrations of RH (0, 10, 20 and 40 μg/mL) for 24 h, total protein was extracted, DR4 and DR5 were detected by Western blot. (C) TRAIL-resistant cells (T24 and BIU 87) were harvested after being treated with different concentrations of RH (0, 10, 20 and 40 μg/mL) for 6, 12, 18 and 24 h. Then total RNA was extracted, and DR5 was detected by qRT-PCR. Differences between groups were examined using one-way ANOVA. The results represent the means ± SD of three replicates, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus control group.