Research Paper Volume 14, Issue 16 pp 6642—6655
Rhein promotes TRAIL-induced apoptosis in bladder cancer cells by up-regulating DR5 expression
Figure 6. Effect of combination treatment RH and TRAIL on cell viability and apoptosis after silencing DR5. (A and B) Three small interfering RNA pairs were designed and verified for interference efficiency with DR5 mRNA (A) and protein expression in BIU 87 cells (B). (C and D) Transfection of TRAIL-resistant BIU 87 cells for 24 h with the siDR5 1, then RH was added. Cell viabilities were detected using CCK8 (C), and apoptosis was detected using Annexin V-FITC/PI (D). Differences between groups were examined using one-way ANOVA. The results represent the means ± SD of three replicates, *P < 0.05, ***P < 0.001.