Research Paper Volume 14, Issue 18 pp 7282—7299

Figure 1. Cisplatin upregulates αklotho expression in MDCK and NRK-52E cells. (A) Arithmetic mean ± SEM of αklotho transcript levels normalized to TBP in MDCK cells treated with cisplatin at the indicated concentration for 24 h (n = 5; Friedman ANOVA followed by Dunn-Bonferroni post-hoc test). (B, C) Arithmetic mean ± SEM of MDCK cell viability (B) or number (C) upon treatment without or with 10 μM cisplatin for 24 h (B: n = 5, one-sample t-test; C: n = 4, one-sample t-test). (D) Rate of apoptosis and necrosis of MDCK cells treated with or without 10 μM cisplatin for 24 h (n = 6, one-sample t test) (E) Arithmetic mean ± SEM of αklotho transcript levels relative to TBP in NRK-52E cells incubated without or with 10 μM cisplatin for 24 h (n = 5, paired t-test). (F, G) Arithmetic mean ± SEM of NRK-52E cell viability (F) or number (G) upon treatment without or with 10 μM cisplatin for 24 h (F: n = 5, one-sample t-test; G: n = 4, one-sample t-test). (H) Rate of apoptosis and necrosis of NRK-52E cells treated with or without 10 μM cisplatin for 24 h (n = 5, one-sample t test) ^*p < 0,05, ^**p < 0.01, ^***p < 0.001 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; ctr: control.