Research Paper Volume 14, Issue 18 pp 7282—7299

Figure 10. Cytostatic drugs and serum deprivation reduce αklotho gene expression and soluble klotho (sKL) protein secretion in HK-2 cells. (A) Arithmetic mean ± SEM of αklotho mRNA levels relative to TBP in HK-2 cells treated with or without 10 μM cisplatin for 24 h (n = 8, paired t-test). (B) Arithmetic mean ± SEM of sKL concentration in the supernatant of HK-2 cells treated with 10 μM cisplatin or vehicle control for 24 h (n = 6, paired t-test). (C) Arithmetic mean ± SEM of αklotho transcript levels relative to TBP in HK-2 cells treated with or without 120 nM paclitaxel (n = 6, paired t-test) for 24 h. (D) Arithmetic mean ± SEM of sKL concentration in the cell culture supernatant of HK-2 cells treated with or without 120 nM paclitaxel for 24 h (n = 6, paired t-test). (E) Arithmetic mean ± SEM of αklotho transcript levels relative to TBP in HK-2 cells treated with or without 300 nM doxorubicin for 24 h (n = 5, paired t-test). (F) Arithmetic mean ± SEM of sKL concentration in the cell culture supernatant of HK-2 cells treated with or without 300 nM doxorubicin for 24 h (n = 4, paired t-test). (G) Arithmetic mean ± SEM of αklotho mRNA levels relative to TBP in HK-2 cells incubated with (ctr) or without 10 % FBS in the culture medium for 24 h (n =5, paired t-test). (H) Arithmetic mean ± SEM of sKL concentration in the HK-2 cell culture supernatant after incubation with or without 10% FBS for 24 h (n = 5, paired t-test). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; ctr: control.