Research Paper Volume 14, Issue 18 pp 7282—7299

Figure 6. Cytotoxic agents and PAC-1 up-regulate apoptotic proteins BAD and BAX in MCDK cells. (A–D) Arithmetic mean ± SEM of BAD transcript levels relative to TBP in MDCK cells incubated for 24 h without or with 10 μM cisplatin (A; n = 5; paired t-test), 120 nM paclitaxel (B; n = 5, paired t-test), 300 nM doxorubicin (C; n = 5, paired t-test), or 10 μM PAC-1 (D; n = 6, paired t-test). (E–H) Arithmetic mean ± SEM of BAX transcripts relative to TBP in MDCK cells treated without or with 10 μM cisplatin (E; n = 5, Wilcoxon signed-rank test), 120 nM paclitaxel (F; n = 5, paired t-test), 300 nM doxorubicin (G; n = 5, Wilcoxon signed-rank test), or 10 μM PAC-1 (H; n = 6, paired t-test). (I–L) Arithmetic mean ± SEM of BAX to BCL-2 mRNA ratio in MDCK cells incubated for 24 h without or with 10 μM cisplatin (I; n = 5, paired t-test), 120 nM paclitaxel (J; n = 5, paired t-test), 300 nM doxorubicin (K; n = 5, paired t-test), or 10 μM PAC-1 (L; n = 6, paired t-test). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; ctr: control.