Research Paper Volume 14, Issue 16 pp 6656—6667

Figure 3. Quantitative RT-PCR gene expression analysis of four genes. The relative expression was calculated using the equation relative quantification (RQ) = 2^−ΔΔCT, and β-actin was used as an internal control. Error bars indicate s.e.m. LM: CRC with liver metastasis, LM-N: CRC without liver metastasis. (A) CRC samples with liver metastasis had a lower mRNA expression of CROP compared to that of the CRC without liver metastasis, but p=0.078 (B) CRC samples with liver metastasis had a lower mRNA expression of NFIB compared to that of the CRC without liver metastasis (p<0.01). (C) CRC samples with liver metastasis had a lower mRNA expression of MALAT1 compared to that of the CRC without liver metastasis (p<0.01). (D) CRC samples with liver metastasis had a lower mRNA expression of NKTR compared to that of the CRC without liver metastasis (p<0.01).