Research Paper Volume 14, Issue 16 pp 6656—6667
Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis
Figure 4. (A) Immunohistochemical staining and assessment of NKTR in primary CRC tissue. (B) Western blot showed NKTR expression in seven CRC cell lines. β-actin was used as an internal control. (C) Western blot showed NKTR expression in DLD1-NKTR-shRNA and mock. (D) Effect of NKTR expression of DLD1 cell proliferation. Error bars indicate s.e.m., n = 3. *P < 0.05, **P < 0.01 (Student’s t-test). (E, F) Effect of NKTR expression on DLD1 cell colony formation. Error bars indicate s.e.m., n = 3. **P < 0.05 (Student’s t-test). (G, H) Effect of NKTR expression on DLD1 cell migration and invasion. Data are representative of each group or expressed as the mean ± s.e.m. of cells per six high power fields. *P < 0.05 (Student’s t-test).