Research Paper Volume 14, Issue 16 pp 6668—6688
MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia
Figure 1. ROCK1 is a direct target of miR-148a in HCT-116 cells. (A) Two putative miR-148a-binding sites in ROCK1 3′-UTR and the two corresponding mutant binding sites (underlined) are shown; (B) miR-148a overexpression suppressed the activity of firefly luciferase that carried the wild-type (P = 0.001) but not mutant 3′-UTR of ROCK1; (C) The mRNA levels of ROCK1 were determined using qRT-PCR in stable HCT116 and HCT116-miR-148a cell lines, and the difference was significant in the HCT116-miR-148a cell line (P = 0.001).