Research Paper Volume 14, Issue 16 pp 6668—6688
MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia
Figure 2. Met is a direct target of miR-148a in HT-29 cells. (A) miR-148a and its putative binding sequences in the 3′-UTR of Met. Mutations were generated at the complementary site (underlined) that binds to the seed region of miR-148a; (B) miR-148a overexpression suppressed the activity of firefly luciferase that carried the wild-type (P < 0.001) but not mutant 3′-UTR of Met; (C) The mRNA levels of Met were determined using qRT-PCR in stable HT29 and HT29-miR-148a cell lines, and the difference was significant in the HT29-miR-148a cell line (P < 0.001).