Research Paper Volume 14, Issue 16 pp 6668—6688
MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia
Figure 3. miR-148a inhibited the expression of HIF-1α and Mcl-1 proteins by directly targeting ROCK1 in HCT116 cells under hypoxia. The protein expression levels of ROCK1, HIF-1α, and Mcl-1 were evaluated under a hypoxic condition generated using CoCl2 and in four cell lines (HCT116, HCT116 + bevacizumab, HCT116-miR-148a, and HCT116-miR-148a + bevacizumab). β-Actin served as an internal control. (A) Protein levels of ROCK1, HIF-1α, and Mcl-1; (B) The protein expression level of ROCK1 was significantly decreased in the HCT116-miR-148a cells but not in the HCT116 cells (P = 0.002); (C) The protein expression level of HIF-1α was significantly decreased (P < 0.001); (D) The Mcl-1 protein expression level was markedly decreased (P < 0.001).