MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia

Hsiang-Lin Tsai; Yueh-Chiao Tsai; Yen-Cheng Chen; Ching-Wen Huang; Po-Jung Chen; Ching-Chun Li; Wei-Chih Su; Tsung-Kun Chang; Yung-Sung Yeh; Tzu-Chieh Yin; Jaw-Yuan Wang

doi:doi:10.18632/aging.204243

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Research Paper Volume 14, Issue 16 pp 6668—6688

MicroRNA-148a induces apoptosis and prevents angiogenesis with bevacizumab in colon cancer through direct inhibition of ROCK1/c-Met via HIF-1α under hypoxia

Figure 4. miR-148a inhibited the protein expression of HIF-1α and Mcl-1 by directly targeting Met in the HT29 cell line under hypoxia. We created a hypoxic condition by using CoCl₂ and evaluated the expression levels of HIF-1α and Mcl-1 in four cell lines (HT29, HT29 + bevacizumab, HT29-miR-148a, and HT29-miR-148a + bevacizumab). β-Actin served as an internal control. (A) Protein levels of Met, HIF-1α, and Mcl-1; (B) The Met protein expression level was significantly decreased in HT29-miR-148a cells but not in HT29 cells (P < 0.001); (C) The HIF-1α protein expression level was significantly decreased (P < 0.001). (D) The Mcl-1 protein expression level was markedly decreased (P < 0.001).