Research Paper Volume 14, Issue 16 pp 6467—6480

Figure 2. BBT-877 reduces glomerular injury in the kidneys of STZ induced diabetic mice. After treatment with BBT-877 for 8 weeks, mice were sacrificed and their kidneys were collected, fixed in 10% formalin buffer, and embedded in paraffin. The kidney sections were stained with hematoxylin and eosin (HE) and Periodic acid-Schiff (PAS). Glomerular surface area was quantified using 30 glomeruli per mouse by ImageJ Software. (A) Representative image of HE and PAS staining, scale bars = 20 μm (B) Glomerular surface area, (C) Serum creatinine, (D) Blood urea nitrogen. Abbreviation: STZ: streptozotocin. STZ-10BBT: BBT-877 10 mg/kg, STZ-30BBT: BBT-877 30 mg/kg, STZ-90BBT: BBT-877 90 mg/kg, STZ-10L: losartan 10 mg/kg. ^*p < 0.05, control-vehicle vs. STZ-vehicle, ^#p < 0.05, STZ-vehicle vs. STZ-10, 30, 90BBT or STZ-10L, n = 8−10.