Research Paper Volume 14, Issue 16 pp 6481—6506

Figure 2. bmm overexpression promotes physiological fitness in both female and male Drosophila. (A) Fecundity of 2-week-old da-GAL4/+ vs. da-GAL4>UAS-bmm female flies. n=168 for da-GAL4/+ and n=154 for da-GAL4>UAS-bmm group. (B) Fecundity of 2-week-old Act5C-GAL4/+ vs. Act5C-GAL4>UAS-bmm female flies. n=93 for Act5C-GAL4/+ and n=84 for Act5C-GAL4>UAS-bmm group. (C) Number of pupae produced by da-GAL4/+ vs. da-GAL4>UAS-bmm female flies at 28 days of age paired with young male w¹¹¹⁸ flies. n=25 for each genotype. (D) Number of pupae produced by w¹¹¹⁸ virgin female flies after paired with da-GAL4/+ or da-GAL4>UAS-bmm male flies at 30 days of age. n=80 for each genotype. Data are shown as mean±SEM and analyzed by two-tailed Student t-test in (A–D). (E) Locomotion analysis of female da-GAL4/+ vs. da-GAL4>UAS-bmm flies at 38 days of age. n=84-151 for each group. (F) Locomotion analysis of male da-GAL4/+ vs. da-GAL4>UAS-bmm flies at 40 days of age. n=147-172 for each group. (G, H) Locomotion analysis of Act5C-GAL4/+ vs. Act5C-GAL4>UAS-bmm at 35 days of age. n=105 for each group. Data are shown as box and whisker plot and analyzed by two-tailed Student t-test in (E–H). (I) Fecundity of 2-week-old inducible da-GS-GAL4>UAS-bmm female flies with or without RU486 induction. n=50 for each group. Data are shown as mean±SEM and analyzed by two-tailed Student t-test. (J) Locomotion analysis of female flies using inducible da-GS-GAL4 driver at 76 days of age with or without RU486 induction. n=60-73 for each group. (K) Locomotion analysis of male flies using inducible da-GS-GAL4 driver at 72 days of age with or without RU486 induction. n=95-109 for each group. Data are shown as mean±SEM and analyzed by one-way ANOVA in (J, K). See also Supplementary Figure 2.