Research Paper Advance Articles

A regulatory circuit of lncRNA NLGN1-AS1 and Wnt signalling controls clear cell renal cell carcinoma phenotypes through FZD4-modulated pathways


Figure 1. NLGN1-AS1 is a novel lncRNA involved in renal malignant transformation. (A) The heatmap of 18 lncRNA expression profiles. (B) NLGN1-AS1 expression in ccRCC tissues and normal tissues from TCGA KIRC dataset. (C) NLGN1-AS1 expression in a series of RCC cell lines (786-O, 769-P, Caki-1, Caki-2, ACHN, A498 and OSRC-2) and human normal renal tubular epithelial cell line HK-2. (D) Representative images of fluorescence in situ hybridization (FISH) assays of the subcellular localization and expression of NLGN1-AS1 (red) while the nucleuses were stained with DAPI (blue) in 769-P and Caki-1 cells. (E) FISH analysis of NLGN1-AS1 (red) in adjacent tissues and ccRCC tissues while the nucleuses were stained with DAPI (blue). The data represent the mean ± SD of 3 replicates. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.001.