Research Paper Advance Articles
Protective effect of fluoxetine against oxidative stress induced by renal ischemia-reperfusion injury via the regulation of miR-450b-5p/Nrf2 axis
Figure 4. Up-regulation of miR-450b-5p aggravated oxidative stress in renal IRI-treated rats. (A) H&E staining of renal tissues in IRI group and IRI+FLX group after the injection of NC agomir and miR-450b-5p agomir at 3 and 21 days after operation in male rats (magnification ×400). (B) Masson staining was used to evaluate renal injury and fibrosis in IRI group and IRI+FLX group after the injection of NC agomir and miR-450b-5p agomir at 21 days after operation in male rats (magnification ×400). (C) DHE staining of renal tissues in IRI group and IRI+FLX group after the injection of NC agomir and miR-450b-5p agomir. ROS exhibit red fluorescence under fluorescent microscope (magnification ×400). (D) Immunohistochemical analysis showed the expression level of iNOS from renal tissues in IRI group and IRI+FLX group after the injection of NC agomir and miR-450b-5p agomir in male rats (magnification ×400). (E–H) Content of MDA, 8-OHdG, T-AOC, and GSH of renal ischemia tissues in each group. Data were presented as Mean ± SD, *significant difference (P < 0.05; **P < 0.01).