Research Paper Volume 14, Issue 18 pp 7455—7469
Low-intensity focused ultrasound attenuates early traumatic brain injury by OX-A/NF-κB/NLRP3 signaling pathway
Figure 3. Low-intensity focused ultrasound (LIFUS) can increase the expression of orexin-A (OX-A) and orexin receptor 1 (OXR1) in traumatic brain injury (TBI) rat brain tissue. (A) OX-A and OXR1 represent western blot protein bands. (B, C) represents the gray values of the OX-A and OXR1 bands, respectively. (D) Representative immunohistochemical staining and relative protein expression of OX-A. (positive expression was brownish yellow. Scale=50 um). (E) OXR1 representative immunofluorescence staining. (OXR1 staining is red, and all nuclear DAPI staining is blue. Scale=50 um). Results are expressed as mean±standard deviation (n=6, SD, *P<0.05, **P<0.01, ***P<0.001).