Research Paper Volume 14, Issue 18 pp 7505—7516

Figure 2. ZCCHC12 promoted OS cell proliferation. (A and B), qRT-PCR was applied to verify the overexpression and knockdown efficiency of ZCCHC12. (C and D), western blot analysis was performed to examine the expression of ZCCH12 in OS cells. (E) CCK8 analyses of the OS cell proliferation with ZCCHC12 overexpression or knockdown. (F) Colony formation assay analysis of cell proliferation after ZCCHC12 knockdown or overexpression in OS cells. n = 5; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.