Research Paper Volume 14, Issue 22 pp 8970—8981
Antrodia Camphorata Polysaccharide activates autophagy and regulates NLRP3 degradation to improve liver injury-related inflammatory response
Figure 3. Suppressing autophagy antagonized the effect of ACP (n=3). (A, B) Flow cytometry results revealed that 3-MA suppressed autophagy, and the effect of ACP was antagonized. Moreover, the cell apoptosis rate in 3-MA+ACP group significantly increased, higher than that of ACP group. *P<0.05 compared with DMSO, #P<0.05 compared with LPS. (C) LC3 staining suggested that 3-MA suppressed LC3 expression, and the fluorescence intensity was weakened. (D) LTG probe detection suggested that 3-MA suppressed lysosome formation.