Research Paper Volume 14, Issue 22 pp 9149—9166

Figure 2. LINC00629 knockdown impaired the anticancer effects of apigenin. (A) LINC00629 was knocked down in UM-SCC6 and Cal-27 cells, and the expression of LINC00629 was analyzed by qRT–PCR. (B–E) UM-SCC6 and Cal-27 cells with or without LINC00629 knockdown were treated with apigenin as indicated. Apoptosis was analyzed by flow cytometry. (F, G) UM-SCC6 cells with or without LINC00629 knockdown were treated with 20 μM apigenin for 36 h. The mammosphere-forming abilities were analyzed. The column charts on the right show the quantitation of spheres. (H, I) The percentage of ALDH1-positive cells was determined in UM-SCC6 cells with or without LINC00629 knockdown. In (A, C, E, F, I) the results represent three independent experiments; *p<0.05, **p<0.01, ***p<0.001.