Research Paper Volume 14, Issue 22 pp 9149—9166

Figure 3. LINC00629 facilitated apigenin-induced Mcl1 decrease. (A, B) UM-SCC6 and Cal-27 cells with or without LINC00629 knockdown were harvested. The protein and mRNA level of Mcl1 were analyzed by Western blotting and qRT–PCR. (C) UM-SCC6 and Cal-27 cells were treated with apigenin at 0 μM or 20 μM for 36 h. The protein level of Mcl1 was determined by Western blotting. (D, E) UM-SCC6 and Cal-27 cells with or without LINC00629 knockdown were treated with or without 40 μM apigenin for 36 h. The protein level of Mcl1 was analyzed by Western blotting. (F, G) Mcl1 was transfected into UM-SCC6 cells with or without LINC00629 overexpression, and the cells were then treated with apigenin as indicated. Apoptosis was analyzed by flow cytometry. In (F) the results represent three independent experiments; ***p<0.001.