Research Paper Volume 14, Issue 22 pp 9149—9166

Figure 5. KLF10 upregulated LINC00629 expression. (A) The altered transcription factors under apigenin treatment in UM-SCC6 cells were selected from the RNA sequencing data and are listed. (B) Overlaps indicating the numbers of transcription factors between RNA sequencing analysis and JASPAR prediction. (C) Heatmap showing the 9 overlapping transcription factors. (D) UM-SCC6 cells were treated with or without apigenin as indicated. The RNA levels of transcription factors were analyzed. (E) EGR1, KLF10 and KLF14 were knocked down using siRNAs. The expression level of LINC00629 was analyzed by qRT–PCR. (F) UM-SCC6 and Cal-27 cells were treated with apigenin as indicated, and the expression level of KLF10 was determined by Western blotting. (G) UM-SCC6 and Cal-27 cells with or without KLF10 knockdown were treated with apigenin as indicated for 36 h. The protein level of KLF10 was determined by Western blotting. (H, I) The expression level of LINC00629 was analyzed by qRT–PCR. In (D, E, H, I) the results represent three independent experiments; *p<0.05, **p<0.01, ***p<0.001.