Research Paper Volume 14, Issue 22 pp 9186—9199

Figure 1. Effects of FRSOD on degeneration, STAS, cytoplasmic calcium level and developmental potential of postovulatory aging oocytes. Restraint-stressed (Strs) and unstressed control (Ctrl) mice were killed at 13, 19 and 25 h after hCG injection to recover oocytes aging for 0, 6 and 12 h, respectively. (A) Shows percentages of degenerated 12 h-aged oocytes. Each treatment was repeated 3 times with each replicate including 60–70 oocytes. (B) Shows percentages of ethanol-activated oocytes after aging for different times. Each treatment was repeated 5 to 7 times with each replicate including 25–30 oocytes. (C) Shows cytoplasmic calcium levels (Ex488/Em515) in oocytes aging for different times. Each treatment was repeated 3 times with each replicate containing 30 oocytes from 2 mice. (D) Shows percentages of blastocysts following Sr2⁺-activation of oocytes aging for different times. Each treatment was repeated 5 to 8 times with each replicate including 25–30 oocytes. (E) Shows rates of fertilization and 2-cell, 4-cell and blastocyst embryos following insemination of 12 h-aged oocytes. Each treatment was repeated 3 times with each replicate including 40–50 oocytes and semen from one male mice. (F) Shows gene expression measured by RT-qPCR in 12 h-aged oocytes. Each treatment was repeated 3 times with each replicate including 30–60 oocytes from 2 mice. Values of control oocytes were set to one (dotted line), and the values in stressed oocytes were expressed relative to it. ^*Indicate significant difference (P < 0.05) from values in control mice.