Research Paper Volume 14, Issue 22 pp 9186—9199

Figure 3. Effects of FRSOD on cortical granules (CGs) distribution and spindle/chromosome morphology of postovulatory aging oocytes. Restraint-stressed (Strs) and unstressed control (Ctrl) female mice were killed at 13 and 25 h after hCG injection to recover 0 h- and 12 h-aged oocytes, respectively. The oocytes recovered were observed under a fluorescence microscope after staining with Hoechst 33342 for DNA detection and with antibodies for CGs or a-tubulin (Tubln) detection. The CGs distribution was classified into normal distribution (NB), mildly abnormal (MA) or severely abnormal (SA) distribution. The spindle/chromosome morphology was classified into focused pole spindle (FS), barrel-shaped pole spindle (BS) or disintegrated spindle (DS) with congressed chromosomes (CC) or misaligned chromosomes (MC). While both FSCC and BSCC were considered as normal spindle/chromosome morphology (NSC), DSCC and DSMC were considered as abnormal spindle/chromosome morphology (ASC). (A, B) Show percentages of oocytes with different CGs distributions after oocytes aged for 0 and 12 h, respectively. Each treatment was repeated 5–6 times with each replicate including 25–35 oocytes from 2 mice. (C) Shows micrographs of oocytes with ND, MA and SA types of CGs distribution. (D, E) Show percentages of oocytes with NSC or ASC spindle/chromosome morphology after oocyte aging for 0 and 12 h, respectively. Each treatment was repeated 5–7 times with each replicate containing 25–30 oocytes from 2 mice. ^*Indicate significant differences (P < 0.05) from values in control mice. The micrographs in (C and F) Were taken at a magnification of 400×. Bar is 15 μm and is applied to all images.