Research Paper Volume 14, Issue 22 pp 9200—9209
Rapamycin improves the quality and developmental competence of in vitro matured oocytes in aged mice and humans
Figure 2. Effects of rapamycin on the quality of IVM oocytes. (A) The immunofluorescence images of spindle and chromosome of IVM oocytes from both groups. Scale bar, 25 μm. (B) The percentages of oocytes with chromosome aberration (n = 86). (C) The percentages of oocytes with abnormal spindle morphology (n = 86). (D) The immunofluorescence images showing JC-1 staining intensity in oocytes matured in different media. Scale bar, 75 μm. (E) The relative levels of MMP (red/green fluorescence intensity) in oocytes from both groups (n = 49). All data were expressed as mean ± SEM from three independent experiments. *P < 0.05.