Research Paper Volume 14, Issue 22 pp 9264—9279

Figure 2. Elevated CD229 expression promotes MM cell proliferation. (A) WB analysis of CD229 overexpression in ARP1 and CAG cells using CD229 and Flag tag antibodies. (B) The proliferation capacity in WT and CD229-OE MM cells was detected by MTT. (C) Flow cytometry revealed that the proportion of G2/M phase was significantly increased in CD229-OE cells compared to WT cells. (D) WB analysis confirmed the reduction of CD229 in ARP1 and CAG cells upon transfection with the siRNA. (E) Decreased CD229 resulted in a lower cell proliferation rate in ARP1 and CAG cells detected by MTT. (F) Flow cytometry analysis revealed that the proportion of G2/M phase was significantly decreased in si-CD229 cells relative to NC cells. The data of experiments represent Mean±SD from at least three independent experiments. **p < 0.01, ***p < 0.001.