Research Paper Volume 14, Issue 22 pp 9300—9316
The roles of CPSF6 in proliferation, apoptosis and tumorigenicity of lung adenocarcinoma
Figure 4. CPSF6 knockdown induced apoptosis of LUAD cells. A549 (A) and NCI-H1299 cells (B) were transduced with lentivirus expressing CPSF6 shRNA (shCPSF6) or control shRNA (shCtrl), labelled with the Annexin V Apoptosis Detection kit, and analyzed on a flow cytometry. Representative images and the apoptotic rates of three independent experiments are shown. ***P<0.001 versus shCtrl. (C). Caspase3/7 activity of A549 and NCI-H1299 cells were detected between five groups after transducing with lentivirus for 5 days. Control cell + control shRNA (NC+NC), CPSF6 shRNA + control shRNA (KD+NC), CPSF6 shRNA + GSK3B shRNA (KD+KD), CPSF6 shRNA + IRS1 Over Expressed (KD+OE1), CPSF6 shRNA + JUN Over Expressed (KD+OE2). **P<0.01, ***P<0.001 versus KD+NC. The number of cell experiments in parallel (n=3).