Research Paper Volume 14, Issue 22 pp 9300—9316

Figure 6. Microarray analysis of A549 cells with CPSF6 knockdown. A549 cells were transduced with lentivirus expressing CPSF6 shRNA (shCPSF6) or control shRNA (shCtrl). (A, B) Disease and function enrichment analyses (A) and canonical pathway analyses (B) with the IPA application. (C) Fold changes of IRS1, JUN, MET, MDM2, ATF2 and GSK3B as indicated by Microarray analysis. (D, E) Real-time PCR (D) and Western blot (E) was performed to validate Microarray data. The densitometric analysis of western blotting was shown below the blot. (F) CPSF6 knockdown-induced changes in the network constructed by IPA. Up-regulated and down-regulated Genes were presented in red and in green, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus shCtrl. The number of cell experiments in parallel (n=3).