Research Paper Volume 14, Issue 24 pp 9860—9876

Figure 1. C-C motif chemokine ligand 2 (CCL2) promotes cell viability, proliferation, migration, and myogenesis in mouse C2C12 myoblasts (MBs). (A) MBs viability on Cell Counting Kit-8 (CCK-8) assay and MBs proliferation on 5-bromo-2′-deoxy-uridine (BrdU) assay. (B) MBs migration on Transwell migration assay. (C) MBs were differentiated into myotubes (MTs) with 2% horse serum after exposure to the indicated concentrations of recombinant CCL2 for the specified number of days. MTs were stained with anti-myosin heavy chain (MyHC) antibody, and the nuclei were counterstained with 4, 6-diamidino-2-phenyindole (DAPI). Scale bars, 100 μm. (D) Quantitative results per field are presented. Number of experiments (No.) of (A, D) = 4 times, respectively. No. of (B, C) = 6 times, respectively. Data are expressed as mean ± standard deviation (SD). (A, B) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. CCL2 '0' (Vehicle: PBS + 0.1% BSA). (C, D) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. Day 1 of CCL2 '0' (vehicle: PBS + 0.1% BSA), or vs. same day data (represented with lines).