Research Paper Volume 14, Issue 24 pp 9860—9876

Figure 6. Effects of C-C motif chemokine ligand 2 (CCL2) on myogenesis are mediated by AKT-mTOR signal stimulation. (A, B) Western blot analyses to determine the activity of signals related to myogenesis in C2C12 cells after treatment with 200 ng/ml CCL2. (C, D) Quantitative reverse-transcription polymerase chain reaction analyses (qRT-PCR) of the relative intensity of p-mTOR/mTOR or p-AKT/total AKT in C2C12 cells treated with 2% horse serum in the presence or absence of recombinant CCL2 and/or CCR2 inhibitor for three days. Number of experiments (No.) of (A–D) = 4 times, respectively. Data are expressed as mean ± standard deviation (SD). (B–D) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. siRNA Control MB, or vs. siRNA control MT, or vs. siRNA control MT & CCL2 (represented with lines).