Research Paper Volume 14, Issue 24 pp 9877—9889
SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production
Figure 5. Proliferation and cell cycle progression are elevated in the SOX2-high MCF7 cells. MCF7 cells stably-transduced with the SOX2-GFP construct were subjected to FACS sorting to isolate the 5% highest GFP (GFP-high) and the 5% lowest GFP (GFP-low) subpopulations. (A) Proliferation was assessed using the xCELLigence RTCA system. In the left panel, a representative tracing is shown. In the right panel, the graph shows the average of the final cell index of at least 3 independent experiments. (B) Cell cycle was evaluated with propidium iodide by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases of the cell cycle are also represented in a tabular format. Experiments were performed at least 3 times independently. Results are shown as the mean ± SD: t-test, two tailed-test, *p < 0.05.