Research Paper Volume 14, Issue 24 pp 10125—10136
Exosomes derived from human umbilical cord mesenchymal stem cells promote osteogenesis through the AKT signaling pathway in postmenopausal osteoporosis
Figure 4. (A) Cell proliferation was evaluated using EdU staining between the control group, PMO group, PMO+Exo group, PMO+bpV(phen) group and PMO+Exo+MK-2206. (B) The EdU-positive cells were calculated using ImageJ. (C) Alizarin red assay was performed to evaluate osteogenesis between different groups. (D) Quantitative calculation of alizarin red-positive areas. (E) We performed an ALP assay to evaluate osteogenesis between different groups. (F) Quantitative calculation of ALP-positive areas.