Clearance of p16Ink4a-positive cells in a mouse transgenic model does not change β-cell mass and has limited effects on their proliferative capacity

Nadine Bahour; Lucia Bleichmar; Cristian Abarca; Emeline Wilmann; Stephanie Sanjines; Cristina Aguayo-Mazzucato

doi:doi:10.18632/aging.204483

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Research Paper Volume 15, Issue 2 pp 441—458

Clearance of p16Ink4a-positive cells in a mouse transgenic model does not change β-cell mass and has limited effects on their proliferative capacity

Figure 2. Inverse correlation between cellular senescence load and proliferative capacity. (A) Overall proliferation quantification of 6–9-month-old INK-ATTAC animals, n_control = 14, and n_treated = 13; (B) Percentage of islets with proliferating cells in 6 and 8/9-month-old INK-ATTAC mice measured by the percentage of BrDU⁺ Ins⁺ cells, n_6month = 15, and n_8–9month = 12; (C) Correlation between percentage of non-senescent FLAG⁻ islets (negative for FLAG staining) and proliferation (positive nuclear staining of BrDU) in 6–9-month-old (n = 19) INK-ATTAC mice. Line of best fit is shown along with dotted lines indicating 95% confidence intervals. P-value was calculated using the null hypothesis that the slope of the best-fit line equals 0.