Research Paper Volume 15, Issue 4 pp 1039—1051
RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinoma
Figure 3. RIOK3 promotes the activation of the mTORC1 pathway via SLC7A2. (A, B) Western blot analysis of RIOK3, SLC7A2, S6, P-S6, S6K and P-S6K in PANC-1 and BxPC-3 cells transduced with shRIOK3-1 alone or in combination with SLC7A2 under conventional culture conditions. (C) The content of Arg was analyzed in the intracellular and culture medium of PANC-1 and BxPC-3 cells transduced with shRIOK3-1 alone or in combination with SLC7A2 under conventional culture conditions. One-way ANOVA test. (D, E) Western blot analysis of RIOK3, SLC7A2, S6, P-S6, S6K and P-S6K in PANC-1 and BxPC-3 cells transduced with shRIOK3-1 alone or in combination with SLC7A2 under arginine-deficient culture conditions. All experiments were performed independently at least three times. The star (*) symbol denotes the level of statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, ns no significance.