Research Paper Volume 15, Issue 4 pp 1052—1073

Figure 4. Overexpression of TIPE2 suppresses the migration and invasion of EOC cells. (A) HeyA8 cell migration was evaluated by the wound-healing assay using Culture-Inserts for Live Cell Analysis. Cells were seeded in culture inserts with a concentration of 3×10^5/ml. After cells had grown to a dense cell layer overnight, inserts were carefully peeled off, resulting in a gap of 500 μm between the cell population. The wounded cell layer was photographed at times 0, 24, and 48h under the microscope. The invasive capability was determined by measuring the reduction in wound area between 0, 24, and 48h in HeyA8 cells (n=3). (B) Inhibition of cell migration in transwell assay. HeyA8 and SKOV3 cells transfected with TIPE2, and its control group was added to the upper chamber of a 24-well transwell. After incubation for 24 hours, the tumor cells on the bottom side of the chamber were fixed and stained with crystal violet (n=3). (C) Inhibition of cell invasion in a transwell assay. HeyA8 and SKOV3 cells transfected with TIPE2, and its control group were incubated for 48 hours in the upper chamber with Matrigel transwell filters from which cancer cells can invade into the lower chamber. Cells on the surface of the lower chamber were fixed and stained with crystal violet (n=3). Data are presented as mean ± SD. Statistical significance is indicated as: **p < 0.01, ***p < 0.001.