**Figure 3.** **Effects of AMPKα isoform reduction on spatial learning and memory in aged mice.** (**A**) The training phase of the hidden platform Morris water maze (MWM) test showed day-to-day escape latency of the mice. n=14 for Cre+/-, n=15 for AMPKα1 cKO and AMPKα2 cKO. *p*=0.78 (interaction between groups). Two-way ANOVA. (**B**) Escape latency on day 3 of the MWM test. **p*<0.05. One-way ANOVA and Tukey’s test. (**C**) Measurement of the target quadrant occupancy in the probe trial phase of the MWM test, n=14 for Cre+/-, n=15 for AMPKα1 cKO and AMPKα2 cKO. *p*=0.056, One-way ANOVA. (**D**) Visible platform test. n=11-15 mice per group. *p*>0.05 One-way ANOVA.