Research Paper Volume 15, Issue 10 pp 4071—4083
lncRNA PITPNA-AS1 promotes cell proliferation and metastasis in hepatocellular carcinoma by upregulating PDGFD
Figure 2. PITPNA-AS1 promotes cell proliferation, migration, and invasion in HCC. (A) PITPNA-AS1 mRNA levels in Huh7 and Hep3B cells transfected with sh-NC, sh-PITPNA-AS1#1, or sh-PITPNA-AS1#2 was determined by qRT-PCR. (B) Proliferation of Huh7 and Hep3B cells transfected with sh-NC, sh-PITPNA-AS1#1, or sh-PITPNA-AS1#2 was measured by the CCK-8 assay. (C and D) Migration and invasion capabilities of Huh7 and Hep3B transfected with sh-NC, sh-PITPNA-AS1#1, or sh-PITPNA-AS1#2 was assessed by the transwell assay. Magnification rate, 200×. (E) EMT-related protein levels in Huh7 and Hep3B cells transfected with sh-NC, sh-PITPNA-AS1#1, or sh-PITPNA-AS1#2 were determined by western blot. **P < 0.01.