Research Paper Volume 15, Issue 10 pp 4144—4158

Figure 6. Exosomal lncRNA GAS5 from hUC-MSCs competitively bind to miR-21 to regulate suppression on target PTEN genes to alleviate EMT of HPMCs. (A) Bioinformatics analysis result showed that lncRNA GAS5 had a binding site with miR-21. (B) Bioinformatics analysis result showed that PTEN had a binding site with miR-21. (C) Dual-luciferase reporter gene assay was used to analyze the relationship between lncRNA GAS5 and miR-21. (D) Dual-luciferase reporter gene assay was used to analyze the relationship among PTEN, miR-21, and lncRNA GAS5. (E) Transfection with lncRNA GAS5 significantly alleviated the HG-induced EMT, however, combination with silencing of PTEN reversed the mitigating effect of lncRNA GAS5 on EMT by Western Blot. (F) Statistical results of EMT changes after transfection with lncRNA GAS5 in combination with silencing of PTEN. (G) The expression of EMT markers was detected by real-time PCR in HPMCs. Each value represents the mean ± SEM (n = 3) (C, D) ^**P < 0.01 vs. Control, ^##P < 0.01 vs. miR-21; (E–G) ^**P < 0.01 vs. Control, ^##P < 0.01 vs. HG, ^%%P < 0.01 vs. HG+ GAS5).