Research Paper Volume 15, Issue 10 pp 4144—4158

Figure 7. lncRNA GAS5 in exosomes from hUC-MSCs-CM alleviating EMT through Wnt/β-catenin pathway. (A) Exosomes from hUC-MSCs could suppress the Wnt/β-catenin pathway in HG HPMCs by Western Blot. (B) Statistical results of Wnt/β-catenin pathway changes after HG and hUC-MSCs/hUC-MSCs exosomes treatment. (C) The expression of Wnt3a and β-catenin was detected by real-time PCR. (D–F) Exosomes from hUC-MSCs could suppress the Wnt/β-catenin pathway in HG HPMCs by transferring lncRNA GAS5 to HPMCs. (G) After transfection with β-catenin vector, alleviated EMT all reversed in HG+ lncRNA GAS5 group by Western Blot. (H) Statistical results of EMT changes after HG and transfection with lncRNA GAS5 and β-catenin vector. (I) The expression of EMT markers was detected by real-time PCR in HPMCs. (J) Exosomes secreted from hUC-MSCs alleviated the transfer into the nucleus of β-catenin in HG-treated HPMCs. (A–F) ^**P < 0.01 vs. Control, ^##P < 0.01 vs. HG, ^%%P < 0.01 vs. HG+ NC GAS5i exo, ^%P < 0.05 vs. HG+ NC GAS5i exo; (G–I) ^**P < 0.01 vs. Control, ^##P < 0.01 vs. HG, ^%%P < 0.01 vs. HG+ GAS5).